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Hello to all dear professors and researchers. I did the electrophoresis part well in the western blot setup phase. But now I have a problem with the same raw materials and the time of electrophoresis to separate the bands is very long. What do you think is the cause and what are the solutions? Thank you very much.
Sabine Strehl
Check your running buffer (salt concentrations, pH); freshly prepare the running buffer; clean the tank and ensure its proper filling; double check that there are no leaks; try to use another power supply and/or tank.
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Katie A S Burnette
"You can't rush quality".
Seriously. It just takes time to separate out the bands. If you turn up the voltage too high, then you will get smeared bands and poor resolution.
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