Why is the concentration of DNA very low after a gel extraction?

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Kyriakos Varypatakis

Hi, losing DNA after gel extraction is expected. To get better results you should be very fast when you cut the band on the UV. Exposure to the UV max 5 seconds, do not expose the gel to the light, and what i usually do is I leave some gel (~2mm) around the band uncut in order to avoid losing more DNA.

I dont think you can do other modifications at this step.

Raheem P K

Thenk you for the answer.I look forward and make changes while doing next time.Thank you for your suggestion Kyriakos Varypatakis