I am using SYBR Gold to assess the thermal stability of viral capsids. Theoretically, as the capsids are heated, the capsid assembly should disintegrate and the encapsulated genome should be released. As the SYBR Gold binds to the released DNA, the fluorescence should increase. Thus, as the temperature increases, the area under the fluorescent curve should increase. This is the case with most of my samples. However, I have one sample that starts out with very high fluorescense, and the area under the fluorescent curve decreases as the temperature increases. I thought perhaps this was due to DNA impurities on the outside of the capsids in this particular sample, but even treating it with DNase before running the SYBR Gold assay does not seem to make any difference. Suggestions are welcomed!
- Badges
- Science topic
- Similar topics
- Chemistry
- Pharmacology
- Medical Pharmacology
- Clinical Pharmacology