At two extreme ends of D2O concentrations IR is generally used but not in intermediate concentration ranges. In intermediate concentration ranges the accuracy is poor. What is the reason?
At intermediate concentrations there occurs a rapid exchange between an infinitesimally small number of (D) and (H) of D2O and H2O and separate (H-O-H) (D-O-D) peaks are not observed.Rather a single very broad peak of much less intensity which encompases both the (H-O-H) (D-O-D) peaks is observed to make exact dermination of nu(max)very difficult,e.g.,accuracy becomes very poor.
In other words: below 1% of heavy water, only negligible amount of D2O molecules exist, practically there are only some H-O-D molecules in H2O and e.g. their O-D stretching can be IR detected in spectral region with lower absorption of H2O. Similarly, above 99% of heavy water, only negligible amount of H2O molecules exist, practically there are only some H-O-D molecules in D2O and e.g. their O-H stretching can be IR detected in spectral region with lower absorption of D2O. Between !% and 99% there are mixtures of D2O, H2O and HDO which are more complex for determination.
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