Why I’m getting line coming down from my agarose well?

24 October 2024 1 4K Report

I’m running 0.8% agarose gel and I had two incidence that the diffusion of the samples and ladder showed a line coming down from the well what could be the reason ?

I run the gel at 75V for 45 minutes and I left it more that an hour to solidify

Libor Krásný

1/ Make sure that your agarose is well-dissolved during the boiling process, without any flakes remaining.

2/ Try lower voltage, e. g. 50 V.

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