Hello,

To summarize: I am silencing a transcription factor in the MDA-MB-231 and MDA-MB-468 cells, and observed alterations in cell proliferation (crystal violet) and viability (resazurin and neutral red) in both cell lines. However, when evaluating cell cycle phases and apoptosis by flow cytometry, no changes in the control and silenced cells are observed in the MDA-MB-231 cells.... But cell cycle was altered in MDA-MB-468 (still to perform apoptosis evaluation in this cell line).

If not cell cycle alterations or apoptosis rate, what could explain the changes of cell proliferation and viability on MDA-MB-231 ?

Any paper that found something related?

Thanks,