Alexandre Luiz Korte de Azevedo

6 Questions 1 Answers 0 Followers

Questions related from Alexandre Luiz Korte de Azevedo

Why I see alterations in proliferation in vitro, but not in cell cycle and apoptosis?

Hello, To summarize: I am silencing a transcription factor in the MDA-MB-231 and MDA-MB-468 cells, and observed alterations in cell proliferation (crystal violet) and viability (resazurin and...

17 August 2024 5,027 1 View

Can qPCR of BAX and BCL-2 transcripts be used to evaluate apoptosis in a general way, or only a western blotting at protein level?

I will perform a apoptosis evaluation in cell lines using flow cytometry after siRNA transfection, and I want to evaluate the levels of BAX and BCL-2 to complement the experiment. However, I can...

10 June 2024 2,160 2 View

How identify ncRNA-coded peptides in mass-spectrometry data?

I have full proteome data (label-free MS/MS) from solid tissue samples of breast cancer and i heve already identified the canonical proteins expressed. Now, I am trying to identify peptides...

21 May 2022 1,424 0 View

How to analyse the results of machine learning of PERSEUS in a ROC (AUC) curve?

Hi everyone. I used the machine learning /cross-validation function of PERSEUS (maxquant) to determine a set of proteins that can discriminate a cancer subtype from the others. The result is a...

07 May 2021 4,196 0 View

How can a super-expressed gene have a inverse correlation with prognostic value?

I am curious about prognostic value analysis: A gene that is known to stimulate metastasis etc.. is upregulated in breast cancer, however, when i make a kaplan-meier analysis to acess the possible...

13 November 2020 5,444 1 View

Hi. I am doing a western blotting investigation, and found in different cell lines bands with different weights... Why this hapenned?

I am using anti-fibronectin antibodies in western blotting experiments, and in one breast cancer cell line, I found ~230kDa bands, but in other cell line of this cancer, I found bands of ~35kDa...

03 July 2020 10,268 3 View