I am working on establishing hairy root culture in Moringa oleifera but i am facing a fungal contamination problem after inoculation with Agrobacterium rhizogens on MS media. what should i do? i am using disposable petri plates afnd my MS media contain cefatoxime antibiotic.
Prosanta Kumar Dash
Hello Maliha!
Possible reasons of fungal contamination in MS media could be-
1. Improperly sterilized media and/equipments.
2. Defect of air filter of laminar flow.
3. Movement of peoples behind you when you worked under hood.
4. No mask on your mouth while you inoculated.
Please double check the above mentioned possible reasons of contamination.
1 votes
0 thanks
Maliha akbar Soomro
Hayat Essalmani Thank you for reply please refer any antifungic for MS media
0 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Physical Sciences
- Materials Science
- Plating
More Maliha akbar Soomro's questions See All
Similar questions and discussions