For example, many phages targeting Gram-negative hosts have been rebooted in E. coli cells. "Rebooting" just means transforming a phage's genomic DNA into a cell in order to engage the host's TX-TL machinery to make phage particles that can then be used to infect their intended hosts.
G+ and G- bacteria have quite a few differences. Many promoters from G+ bugs don't work in E. coli, lysis mechanisms will be different (one membrane instead of two) and so on. So while I don't know if anyone has tried it or not, I can imagine that in many or most cases it would not work.
Why not? Curtobacterium phage Ayka (Tarakanov, R.I.;Lukianova, A.A.; Evseev, P.V.; Pilik,R.I.; Tokmakova, A.D.; Kulikov, E.E.;Toshchakov, S.V.; Ignatov, A.N.;Dzhalilov, F.S.-U.; Miroshnikov,K.A. Ayka, a Novel CurtobacteriumBacteriophage, Provides Protection against Soybean Bacterial Wilt and Tan Spot. Int. J. Mol. Sci. 2022, 23,10913. https://doi.org/10.3390/ijms231810913) is similar to Rhizobium phage RHphN3_8 infecting Gram-negative Rhizobium (NCBI Reference Sequence for phage: NC_073480.1)
The article below has described Clavibacter michiganensis (gram+) Phage33 that was clustered together with bacteriophages of Xylella, Stenotrophomonas and Xanthomonas.
Bekircan Eski D, Gencer D, Darcan C. Whole-genome sequence of a novel lytic bacteriophage infecting Clavibacter michiganensis subsp. michiganensis from Turkey. Journal of General Virology. 2024 Jul 15;105(7):002006.
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