In our lab, we perform isolation of phosphorous solubilizing bacteria from soil on Pikovskayas meida (Serial dilution method). When morphologically identified zone forming gram positive bacteria are picked and re-streaked on pvk meida, they grow on plates but completely lose their ability of zone formation. What kind of media modification or technique should I use to retrieve their ability?
Subculturing of bacteria in natural medium ( without essential substrate) Leading to gradual losing their ability to solubilizing phosphorus... good luck
Hope this will help you Article Isolation and Characterization of a Phosphate-Solubilizing H...
Phosphate-solubilizing bacteria (PSB) can promote the dissolution of insoluble phosphorus (P) many facter effects activity and solubilized it specificly type medium,,,,,,
I have the same problem. Do you have now the technique to retrieve it? Thank you.
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