This plasmid has been growing on LB broth + Carb however it is not responsive now
What can be a hypothesis related to the Stock out Cost or Shortage Cost?
29 July 2024 9,259 2 View
Hi, I need to prepare a standard curve of H2O2 to evaluate the primary products of oxidation. H2O2 I have is 35%. How do I prepare a stock of H2O2 10 microlitere. If s.o has experience, I would be...
26 July 2024 3,460 2 View
I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...
22 July 2024 6,592 3 View
Hi ResearchGate community, how are you? I need to request your help to mitigate the generation of microbubbles detected during the slide preparation of sample metaphase chromosomal spreads. I...
01 July 2024 7,694 4 View
Bonjour, j'ai une question si quelqu'un a une réponse. J'ai une colonne Uptisphere Strategy 100 Å Bonding HILIC-HIT avec une taille de particules de 3 µm et une longueur de 100 mm. Je n'arrive pas...
24 June 2024 7,201 1 View
The emerging markets have suffered from a state of stagflation. I hope you know your expectations according to your experiences regarding the implications for the stock markets,,,, risks and...
19 June 2024 4,410 3 View
I saw the iPSC about a week after holding the stock (12 well and 3 stocks) and there were very few cells attached, and some cells were differentiated. I wonder what the problem is. The plate is...
12 June 2024 6,087 0 View
how avoid forming lumps in disolving Laemnili buffer 5x? due to presence of undissolved bromoclesol blue and thick glycerol. Please tell me how to resolved this issue.
12 June 2024 10,034 3 View
I am working on antimicrobial effect of CBD (Cannabidiol). As it is insoluble in water, I prepared a stock of 500 mg/ml (in DMSO). Now I have two problems: 1- I can not dissolve more than 500 mg...
10 June 2024 9,564 6 View
I've pelleted down the overnight ampicillin supplemented cultures of DH5ALPHA for pET22b isolation 5 days ago and stored them on -20°C without adding any buffer or glycerol. Would the cells be...
09 June 2024 6,479 2 View
