When we use gel extraction clean-up kit, the yield of nucleic acid mostly reduced i. ie we lose concentration? I,ll be grateful if you can provide any reference support this issue please.
Hi all, Currently, I electrodeposited polypyrrole on carbon electrode but I got some problem when polypyrrole also deposited on the surface of the counter electrode. The concentration of pyrrole...
05 February 2021 2,103 3 View
Dear Respectable Scientists, I have been trying to extract DNA from above mentioned sources using different CTAB/SDS Buffer Compositions and purifying agents but I am not able to extract DNA from...
12 January 2021 5,899 8 View
I have a dataset including Categorical variables and involves multiple response variable. 20% of the cells in cross tab has less than 5 frequencies and hence I cannot carry out a Chi-square Test....
26 December 2020 4,180 3 View
Hello fellow researchers around the globe :) Happy Holidays!! We are conducting a survey on understanding how device usage during everyday life has developed amid the Covid-19...
21 December 2020 5,363 4 View
I know that this system was regional before, thus covering specific areas not alla the global
09 December 2020 3,343 3 View
Educators around the world face a new challenge of how to conduct exams to assess what students have learned after the shift towards e-learning following the closure of nearly all universities...
21 September 2020 4,180 15 View
ith the development of Internet technology, online examination has become more Polar because it helps people save a great deal of energy and time. As an effective and efficient method of teaching...
21 September 2020 2,814 12 View
I have to calculate heat transfer co-efficient on shell side and plot its variation along the length of heat exchanger
07 September 2020 9,078 6 View
I am simulating shell and tube heat ex-changer i have mass flow inlet and pressure oulet (zero gauge) but my pressure readings are not accurate i have tried velocity inlet but results are same....
07 September 2020 7,298 11 View
hello dear microbiologist Am kinda new and still learning SPSS I want to know what kind of tests should I run for AST results I see lots of paper use chi square. And can anyone provide me with...
10 August 2020 9,911 4 View
I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...
03 March 2021 3,661 2 View
I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...
02 March 2021 4,443 2 View
Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...
01 March 2021 8,766 3 View
I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??
28 February 2021 2,153 1 View
hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...
27 February 2021 3,882 3 View
I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...
27 February 2021 9,176 3 View
I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...
25 February 2021 8,140 3 View
I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...
25 February 2021 2,225 5 View
I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...
24 February 2021 8,325 1 View
I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...
24 February 2021 6,310 3 View