Why gel extraction clean-up kits of nucleic acid yielded low quantity of eluted product?

07 July 2018 0 10K Report

When we use gel extraction clean-up kit, the yield of nucleic acid mostly reduced i. ie we lose concentration? I,ll be grateful if you can provide any reference support this issue please.

Badges
Science topic

Similar topics
Gels

More Labeed A. Al-Saad's questions See All

PhD thesis topic?

I want to write a topic for my PhD thesis in hospitality (hotels), can u please suggest some variables

29 July 2024 9,058 3 View

How to converge PEMFC in Ansys Fluent?

I am simulating 1 cell of PEM with an area of 50 cm². The mesh has an orthogonality of 0.2 for the channels and bipoar plates (polyhedra) and a quality of 1 for the active zones (hexagonal). The...

26 June 2024 2,358 0 View

The impact of AI analytical tools?

I am currently conducting a study on the impact of AI analytical tools. Your insights would be incredibly valuable to this research. Would you be willing to spare just a few minutes of your time...

05 April 2024 2,917 1 View

How to apply wavelet transform on real signals from protection relays ?

How to apply wavelet transform on real signals from protection relays ? in matlab simulation the signals are sended to workspace and then the WT is applied but how to apply it to real signals...

02 April 2024 2,653 4 View

What is the role of artificial intelligence in psychological and social crises?

As technology races forward, AI is proving to be a powerful resource in tackling mental health and social issues. Early Warning System: AI can analyze data to predict mental health crises before...

29 March 2024 2,425 2 View

What are the main sport sudden death causes?

Many athletes face sudden death in football or Olympic games without know the causes or misunderstanding to prevent them

27 March 2024 6,257 1 View

How can I get more cloud storage space on my devices, knowing that I have a university email, but my university only gives 100GB?

I don’t have enough space.

27 March 2024 9,707 0 View

Music therapy what is the new?

Dose music therapy still benfit as adjustment management in cancer treatment

26 March 2024 6,122 3 View

What methodologies and technologies are most effective for building interactive chatbots with high responding accuracy?

I am conducting research on the development of interactive chatbots that prioritize accuracy in their responses. My aim is to gain insights into the most effective methodologies and technologies...

17 March 2024 333 3 View

heat stress-chicken ?

Stress has a significant influence on the productivity of poultry, resulting in negative impacts. To reduce these impacts, various procedures and additions have been designed. As researchers, we...

25 February 2024 9,018 0 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

05 August 2024 3,246 1 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to check pH of a high strength hydrogel?

We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...

30 July 2024 942 2 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View