Why fluorescent polystyrene beads stick to PDMS micro channels?

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Navid Yarahmadi Popular answer

Hi Guys,

The problem solved by adding some tween 20 as surfactant, also this problem was not observed using biological cells, thanks you in advance.

Yusuf Kahraman

Have you tried to treat pdms with silane?

Navid Yarahmadi

salam Yusef ,

thanks for your answer, I don't think silane can helps since it makes PDMS more hydrophobic , although hydrophobicity is desirable in SU-8 molding but it is the main reason of non specific protein adsorption.

فی امان الله

Hi John

thanks for comprehensive answer and useful links, Pluronic F127 is widely available in Iran and relativity cheap I'll check its feasibility and inform you about the results.

Thanks for your attention