Good day,
I am doing degradation study of bone scaffold (alginate and HAP) using SBF solution. The scaffold was immersed about 1 month and the solution was refreshed twice every week. Finally, I rinsed it and dry.
Supposedly, the final weight should be lesser than initial. But not in my case. The final weight after dry was higher compared to the initial. Does this mean no degradation process occur? Why this happened?
Yes @Ramom Mezari, there is calcium phosphate deposition on the surface. From what I read, researchers study the degradation rate using SBF solution. Their final scaffold mass is lower than initial, indicate the degradation process. But my results were opposite. I am not sure why?
SBF contains some ingredients which deposit on the scaffold. Beside, HAP and alginate scaffold could be degraded in SBF, however they can deposit on the scaffold too. Weighing the raw scaffold and degraded scaffold wasn't a accurate method. Why don't you use GPC to evaluate the degradation of these samples?
Hi Vu Viet Linh Nguyen , thank you for your explanation. May I know what is GPC?
https://en.wikipedia.org/wiki/Gel_permeation_chromatography
you can compare the number average molecular weight (Mn) and PDI of these samples. remember, clean the scaffold with Distil water and freeze dry before using GPC. Good luck
Dear Saeed Samani , thank you for your great explanation. I will consider your suggestions properly.
Thank you.
this is happening because of the deposition of the HAp layer as well as NaCl deposition. to overcome this problem follow
1. change the SBF solution every 2nd day
2. first remove the SBF-wash the scaffold properly and again fill the SBF solution
3. keep the proper volume of SBF for degradation of scaffold
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