Why double band appeared on TLC plate?

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Md Sahadat Hossain Popular answer

Well side @Tanwir Athar

Gunawan Indrayanto

Hi, Did you have pure standard? Did you mean that your standard appeared as 2 bands (or two spots)? Please check the spectra UV/VIS of the two bands, check also the purity of those two bands. It could be your standard was not pure compound.

Hendy Suhendy

- Make sure the purity of the standard compund using 2D TLC

- Check also contaminant compund on the solvent