I need to perform a motility test for candida albicans and s. epidermidis. However, they do not reduce the TTC in the motility agar. I have come to find out that TTC only works on gram negatives, or atleast it works best for gram negative. Is there another way to visualize the motility of these bugs?
Motility by the bacterium is mostly demonstrated in a semi-solid agar medium. In semi-solid agar media, motile bacteria ‘swarm’ and give a diffuse spreading growth that is easily recognized by the naked eye.
Kindly check the following link:
https://microbiologyinfo.com/motility-test/
Dear Dr. Amir Kalani, I think the information provided by Dr. Manal Hadi Kanaan is very useful. Thank you both for this information sharing discussion. Warm regards Yoganandan G
Sorry, I can't answer the question. TTC like all media has limitations. It isn't a matter of Gram + or – not all organisms cause the colour change and can't be guaranteed to be used to detect motility in the media. Microscopic examination and the sensible answer from Manal Hadi Kanaan can provide better information.
One of your problems will be that neither Candida albicans nor Staphylococcus epidermis are motile, so your results should be negative
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