Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

Explain plasmid description?

Hello everyone, I got a plasmid containing E. coli, and its description on the tube is Bacterial stab (DH5a)_HlZ31_cr_Amp_, I know that Bacterial stab refer to LB medium, DH5a is the name of E....

14 January 2021 6,326 2 View

How big the Balb/c mice spleen after immunization?

I am working with Balb/c mice, and in my experiment, I have to inject mice with antigens, then get their spleen and collect the splenocytes for fusion. Recently, I get into trouble with the mouse...

04 January 2021 3,591 3 View

Capture of oleophilic solid polymers present in water solution ?

I am looking for articles, coferences, publications or thesis concerning the capture of solid oleophilic polymers in solution in water. Thanks Thierry Le Blan

01 January 2021 5,798 3 View

Can I do gene expression with partial cds (not the complete one)?

Hello everyone, I got a partial cds, and I want to express it in yeast, does it work with partial cds or I need a complete one? If partial cds is possible, can you please suggest me some articles...

27 December 2020 3,688 3 View

Hybridoma stops producing antibodies after thawing from liquid nitrogen?

Dear scholars, Our research is currently involved with hybridoma technology. We have isolated some original clones after fusion and we are preparing the subclone step to find some true positive...

01 December 2020 1,178 3 View

Suggestion for normal ovary cell lines?

Dear all, I want to choose a normal ovary cell line and check for its proliferation property when introducing carcinogenic factors. Does anyone have any suggestions for the cell line? Many thanks...

08 November 2020 9,100 3 View

What is Snapback Primer in realtime PCR?

Dear all, I find a paper using Snapback primer. I do not understand the function of snapback tail? What is snapback primer? When do we use snapback primer? and why we use that? Thank you.

01 November 2020 8,869 2 View

Rotation matrix - 3D dimension to 2D dimension?

22 October 2020 9,354 4 View

Am I introducing any potential loss of RNA integrity by combining an RNALater solution with a non-ionic detergent?

I am planning to characterise the transcriptome of pre-anthesis kiwifruit anthers. The samples are being harvested on my behalf and sent down by post, and I cannot ask the harvesters to dissect...

10 October 2020 1,830 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

Any Differences in COVID-19 vaccines?

Are there any differences (outside of the clinical trials that led to their EUA) observed in the rate of infection among those who have received the Pfizer vs Moderna vaccine since the mass...

22 February 2021 9,727 6 View

How to find natural frequency of a bridge from vehicle induced vibrations?

i have frequencies of bridge that are effected by mass of vehicle. the problem is when any vehicle pass the bridge it would add the its mass to the bridge mass ultimately mass of the bridge will...

22 February 2021 10,004 3 View

Did any urologists have experienced with retroperitonel leiomyosarcoma from renal vein with IVC enxtension?

Recently, we got one lady with right side retroperitoneal mass involving renal vein and extends into IVC upto diaphragm. CT suggested leiomyosarcoma. Right kidney separately seen from mass and it...

19 February 2021 6,873 1 View

How can I import .sav file into LISREL?

I've just downloaded free trial version of LISREL 10.3 but I got stuck with importing .sav (IBM SPSS 25.) file. I mainly followed the steps of this video...

15 February 2021 752 2 View

How do you interpret theoretical vs. experimental reaction orders with respect to a reactant?

I recently came across a paper focusing on the hydrodeoxygenation of oxygen-containing compounds. The paper linearized the rate equation in order to determine the experimental reaction order with...

14 February 2021 8,254 2 View

Can i use the " weight moisture" ( humidite ponderale in French) of plants to describe their efficiency in using water?

Weight moisture is : water content/ dry weight , if for example it is 4 it means that this plant have for each 1gam of dry mass 4 grams of water. can i use this to interpret the plant use of...

14 February 2021 5,784 1 View