What is the suitable date of FITC-DMSO solution using?

Hello I want to know if I make a FITC-DMSO solution, how long time I can use it? Can I use the solution after a week? thanks for your help

08 January 2021 1,506 3 View

Why nan loss values are resulted through deep learning in python?

hello every one i want to practice deep learning as descibed for a home price prediction problem i use code as following # IMPORTING PACKAGES import pandas as pd # data processing import numpy...

07 January 2021 8,761 1 View

How can I find decay time from time resolved fluorescence curve?

Hello I put a sample in the Time Resolved Flourescence. Then the instrument gave me a time-intensity curve. Now I want to find the decay time of the sample. If you know anything about these,...

03 November 2020 1,012 4 View

How can i reduce the vegatation effect in sattelite images for mineral exploration tasks?

hello everyone, i want to examine the mineral distribution for exploration purposes using sattelite images (ETM, Aster, hyperion etc.) but how can i reduce the vegetation effect on my pocessing...

23 August 2020 716 4 View

IEEE-10 Generator 39 Bus System( simulink in matlab)?

Hi. I need IEEE-10 Generator 39 Bus System( Simulink in MATLAB). If you have it please share it with me.

14 March 2020 2,989 2 View

I need a trick to remove DNA contamination from extracted RNA samples?

Hi, everyone I am extracting a total RNA from rat brain samples for real-time PCR studies, but unfortunately, there is DNA contamination in gel electrophoresis. I tried to remove it and I failed....

05 October 2019 897 6 View

Cancer cells and gene expression databases?

Hello, I'm trying to find the specific gene expression in various types of cancers and cancer cell lines, Is there any database in this regard?

25 September 2019 6,535 10 View

The reason why rats die in targeting both substantia nigra in LPS model of parkinson's disease?

24 July 2018 7,976 3 View

Does anyone have a finite difference Matlab code for nonlinear one dimensional ground response analysis ?

for multi layered soil - as it has been explained in Kramer's book

24 April 2018 5,081 3 View

Cooperation invitation to establish a website?

Hi, A group of engineers are establishing a website that offers video tutorials for different engineering software including but not limited to Abaqus, Ansys, Catia and so on. They already have a...

22 December 2017 8,754 2 View

Fluo 3-AM in pancreatic beta-cells?

Dear all, I'm trying to understand if a molecule of my interest (capable of inducing glucose-stimulated insulin secretion) determines an increase in intracellular calcium levels in the presence of...

16 February 2021 453 2 View

Why daily dexamethasone given to rats fed high fat/fructose/salt cause very high mortality rate?

rats were given daily dexamethasone in drinking water corresponding to 0.5 mg/kg or 2 mg/kg. These rats are simultaneously fed 25% fat, 3% salt in diet and 10% fructose in drinking water but...

15 February 2021 7,634 2 View

What is the possible cause of MSCs low growing?

Hello Everyone, We are inexperienced in culture of MSCs trying to grow a sample from BM of a mare. It is the second time that after preparation of new medium (DMEM-F12 with high glucose - 10% Hy...

07 February 2021 9,989 7 View

Need for help. What goes wrong with my differentiatio process of C2C12?

Hello, I'm now working on C2C12 myoblast cell line. Growing the cells was great, but there are some problems during differentiation process. The cells are not differentiating but frequently...

30 January 2021 9,535 2 View

Difficulties purifying Outer Membrane Vesicles using a Sucrose Density Gradient: Unable to detect any protein after this process?

I'm currently trying to purify Outer Membrane Vesicles (OMVs) following their extraction from the bacteria I'm working with. After the initial extraction the concentration of protein seems good...

27 January 2021 6,852 3 View

How to simulate enzymatic hydrolyis of sucrose in a CSTR coupled with ultrafiltration membrane with comsol?

Dear all, I am trying to simulate the enzymatic hydrolysis of sucrose with invertase in CSTR in Comsol. The reaction pathway is such that Sucrose + Water -> Fructose + Glucose. It is desired...

27 January 2021 2,525 4 View

How to design an Ultrafiltration membrane for a CSTR?

Dear all, I am designing an Ultrafiltration (UF) membrane coupled with a CSTR. It is desired to utilized a dead end stirred reactor. The main purpose is to separate the enzyme used from the final...

27 January 2021 7,924 1 View

About protocols used for glucose uptake in HepG2 cells?

Hello everyone. I am curently working on a protocol to measure glucose uptake in HepG2 cells using 2-NDBG. The work involves the pre-treatment of cells with an active material and observe if it...

25 January 2021 7,608 3 View

Using a fixed angle rotor with Sucrose gradient /cushion ultracentirfugation?

I am trying to fractionate virus from plasma into different densities by sucrose gradient but we only have fixed angle rotors... Has anyone tried doing that with a fixed angle? Should I use the...

13 January 2021 8,372 3 View

Does anybody have the recipe for making a home made R electroporation buffer for Neon transfection systems?

I tried the recipes below in two cells lines: 1.DPBS 2.DPBS+MgCl2(1mM) 3.DPBS+Sucrose(250mM) 4.DPBS+MgCl2(1mM)+Sucrose(250mM) 5.R Buffer (Neon) the results showed that R buffer is the best one in...

06 January 2021 6,776 1 View