Dear all,

recently, I observed by fluorescence microscopy that a recombinant Antibody Fragment opted for secretion amasses in the late(?) Golgi.

Producer cell Line is CHO Derivative K1.

What may be the reasons for the failed secretion? What are Options to rescue secretion?

Maybe some experienced Scientist in mammalian Expression migh Point Out possible reasons and potential workarounds?

Thank you all in advance!