I noticed most of Blank spectra of solvents used to solubilize my sample show high excitation bands (>700 arbitrary unit), but low emission band (
Might i ask what the solvent is that you are using to solubilise your material?. Secondly, make sure the settings are correct on the UV-Visible spectrophotometer i.e you are actually running a blank to remove the solvent from the spectrum and not producing the spectrum of the solvent plus the material. If you have set up the machine correctly and run the blank as the machine require you to do and the problem is still occuring, i would consider this a problem and check for contamination in the solvent. Try using a fresh bottle and see if this solves the issue.
Since you mentioned excitation and emission, I assume you are doing fluorescence spectroscopy, not absorbance spectroscopy. The most likely reason for a high signal in the excitation is an insufficient separation of the excitation and emission wavelengths so that you are getting a stray light signal. Another likely source is Raman scatter.
Hannah, I am using about 18 common solvents. most of them shows high band in excitation spectra only. I collect the spectrum of the solvent alone (Blank), then of my compound solubilized in solvents giving order to the fluorometer to subtract the blank spectrum.
Adam, I am doing both absorbance and fluorescence spectroscopy. My exc is at 350 and em at 450. my question is this high excitation band of most of the solvents used, affect the accuracy of my measurements ?
You just need to subtract the absorbance or fluorescence of the solvent alone from that of the sample dissolved in it in order to get the spectrum of the sample.
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