01 January 1970 0 5K Report

I am purifying a specific antobody from immunized-rabbits. After HPLC purification, antobodies were functional but the concetration was low. So, I used an AMICON ULtra 4 centrifugal filters ( cut-off 100KDa from MERK) following manufacturer's instructions to concentrate and also change the buffer. The concentration of concentrated antibody could not be determinated by Bradford method, but neither in the buffer under the filter. I proceeded to assay antibody activity in the solution from both compartments and I had not activity.

I suspect that antibody were retained in the filter.

Could you help me? Thank you!!!

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