Why do we potyvirus clone in fragments (by subcloning) and not full length genome at a time?

06 May 2014 1 1K Report

I think the genome size of potyvirus is ~10 kb but how does this large size hinder cloning? How can we construct an infectious clone of potyvirus?

Vincenza Ilardi

You can start looking at this paper:

Maiss, E., et al. "Infectious in vivo transcripts of a plum pox potyvirus full-length cDNA clone containing the cauliflower mosaic virus 35S RNA promoter." The Journal of general virology 73 (1992): 709-713.

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