Lalit Meena

5 Questions 12 Answers 0 Followers

Questions related from Lalit Meena

Can we perform polysaccharide oxidation in phosphate buffer at neutral and alkaline pH?

Basically I am trying to do oxidize the branched polysaccharide but in acidic condition its aldehyde groups are attacked by neighbor hydroxyl group and blocked the functional aldehyde group in...

27 January 2015 2,080 8 View

How can we increase the cross linking between -OH (hydroxyl) and -CHO (aldehyde) groups?

Actually I am trying to cross link my hydrophilic (-OH containing) polymer with higher chain length cross linker (-CHO containing), but I didn't get enough cross linking density to maintain the...

23 January 2015 9,910 2 View

Hi, I want to clone the full length of potyvirus which is a positive single stranded RNA with a size of 9.7 kb. How can I clone it?

Since the size is a bit larger (9.7 kb) I have to clone it in fragments (subcloning). Overlap primers can be designed for PCR amplification.

30 December 2014 608 3 View

Why do we potyvirus clone in fragments (by subcloning) and not full length genome at a time?

I think the genome size of potyvirus is ~10 kb but how does this large size hinder cloning? How can we construct an infectious clone of potyvirus?

06 May 2014 1,307 1 View

Can anybody tell me what refractive index I should use for chitosan nanoparticles size and zeta potential analysis by Zetasizer?

I want to characterize my NP formulations by Zetasizer but I don't have exact knowledge about refractive the index for chitosan NPs. If anybody has some information regarding this please share.

02 March 2014 3,581 1 View