Lalit Meena

3 Questions 7 Answers 0 Followers

Questions related from Lalit Meena

Can we perform polysaccharide oxidation in phosphate buffer at neutral and alkaline pH?

Basically I am trying to do oxidize the branched polysaccharide but in acidic condition its aldehyde groups are attacked by neighbor hydroxyl group and blocked the functional aldehyde group in...

27 January 2015 2,039 8 View

Hi, I want to clone the full length of potyvirus which is a positive single stranded RNA with a size of 9.7 kb. How can I clone it?

Since the size is a bit larger (9.7 kb) I have to clone it in fragments (subcloning). Overlap primers can be designed for PCR amplification.

30 December 2014 570 3 View

Why do we potyvirus clone in fragments (by subcloning) and not full length genome at a time?

I think the genome size of potyvirus is ~10 kb but how does this large size hinder cloning? How can we construct an infectious clone of potyvirus?

06 May 2014 1,274 1 View