What are the pretrained-language-model that is obviously better than BERT and RoBERTa?

19 January 2021 602 2 View

Can I design an aptamer that can sense microbial activity?

I am trying to design a boron nitride based biosensor that can sense the abnormality of microbial activities on fish skin. I am very curious that is it possible to have such an aptamer that can...

23 September 2020 6,352 4 View

Can I build a stable cell line for CO-IP?

I want to know two protein interactions via CO-IP, and one of the proteins has several different mutations. I need to transfect all these mutations into HEK293T cells for doing Co-IP every time,...

03 September 2020 342 3 View

Based on transformer, how to improve the text generation results?

If I do not pretrain the text generation model like BART, how to improve the result based on transformer like tensor2tensor? What are the improvement ideas for transformer in text generation task?

18 August 2020 7,265 3 View

Are there any deep models that are better than BERT-CRF in NER task?

Named entity recognition (NER) is task that mark tags of the input text sequence. BERT-CRF is a good NER model. I want to find a better NER model. Or I want to improve the BERT-CRF model. What...

18 August 2020 7,070 2 View

What are the effects of gender of performer on animal?

I remember a paper studied the effects of gender of handler on animal (mouse?) showing that male handler has more negative effects on animal (for example, causing animal anxious) compared to...

05 August 2020 4,131 1 View

Question of pretraining text-generation task, it seems that pretraining is not work for a small model?

My task is to generate keywords from sentences. I pretrain a text-generation model. I mask the sentences' tokens and predict the whole sentences' tokens. Pretraining batch_size = 8 and step =...

28 July 2020 5,675 1 View

How to tackle endogenous problems with "truncated" data structure?

When I want to estimate educational return, I run a regression like: ln(wage)=beta1+beta2*education+other control variables+u In order to tackle the endogenous problems induced by potential...

21 June 2020 2,244 1 View

What is the principle of Unsupervised Data Augmentation (UDA)? Why does UDA work?

UDA(https://github.com/google-research/uda) could achieve good accuracy by only 20 training data on text classification. But I find it is hard to reproduce the result on my own dataset. So I...

05 June 2020 1,486 2 View

What are the simplest methods for the label noise problem?

If I have enough low quality data from unsupervised methods or rule-based methods. Do you think removing the wrong data predicted by trained model is a simple but effective method?

02 June 2020 4,078 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View