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Questions related from Yiling Guo
Hi, I've been having problems working with small organoids (around 100μm in diameter) for immunostaining. Whether I'm passaging them or fixing them for imaging, I consistently face the issue of...
30 November 2023 6,469 3 View
If we are culturing organoids in matrigel, do we really need to remove matrigel for organoid (confocal) imaging?
30 November 2023 1,127 2 View
It just came cross my mind - if matrigel is derived from mice, would there be an effect on the human organoids that we are culturing/generating? If so, how can we measure the changes? is there a...
23 October 2023 412 4 View
I was always told that after lysing the cells, spin them, and only take the supernatant or storage, but why? why do we need to spin the cell lysis to remove the pellet?
21 June 2023 1,715 1 View
I assume cells will become cells that you want when cultured in the special media containing different growth factores and inhibitors. BUT what would happen to those dead/'irrelatvent' cells?...
01 January 1970 7,357 0 View
