Why do we need to block endogenous biotin during the IHC technique? And what is the benefit of the HRP?

Amira Abou El-Nour @Amira-Abou-El-Nour-2

02 November 2023 0 9K Report

This could give us false positive results, but my question is, how does this happen? Does the antibiotic mix it with biotin by mistake?

Badges
Science topic

Similar topics
Phytochemistry
Extracts

More Amira Abou El-Nour's questions See All

Do you think can be any Uranium bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about Uranium ore deposits in world.

11 August 2024 6,720 0 View

Do you think can be any diamond bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about diamond ore deposits in world.

11 August 2024 2,167 1 View

What is the difference between mathematical R^4 space and physical 4D unit space?

We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...

10 August 2024 6,678 14 View

If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?

10 August 2024 8,198 5 View

Controlling for pupil light reflex when analyzing pupil size time course?

I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...

10 August 2024 3,229 0 View

What are a “Farmers Producer Organization” (FPO) and its essential features?

10 August 2024 477 5 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,291 2 View

How to get moment output in Abaqus Standart?

I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...

08 August 2024 4,831 4 View

How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?

08 August 2024 8,162 0 View

How to calculate FIC index?

Can someone explain to me how was the MIC of MO in combination and MIC of antibiotic in combination was calculated? as shown in the attached file below.

26 July 2024 2,702 1 View

How do I avoid HEK293 cell lifting during biotinylation?

I’m doing cell-surface biotinylation with HEK293 cells and after all the washing and biotin incubation steps (washing with PBS ph8, neutralizing unreacted biotin with 40mM Tris ph8) many of my...

23 July 2024 4,740 3 View

Are those seeking solution part of the problem?

In reference to antibiotic as a commodity, what is your take and way forward on common molecules in both Veterinary and Human antibiotics in relation to currently experienced emergency of AMR?

20 July 2024 4,020 0 View

Is perfusion required to stain brain tissue with every method?

I'm looking to immunostain some brain tissue collected previously. The animal was *not* perfused prior to tissue collection. Will the presence of blood interfere with all staining methods...

14 July 2024 177 4 View

Does it work as antibiotic resistance if plamid has no RBS??

I am on gene work to express gene of interest in some Bacillus species. Thus far, i need to try some RBS in Vector which is optimized for bacillus subtilis. Before i design vector to express in...

11 July 2024 7,847 1 View

Is antibiotic bad for transfection?

I'm going to transfect cells with expression plasmids and later siRNAs against the same gene. Is it true that penicillin/streptomycin in the media negatively affects these processes? Shout I use...

07 July 2024 8,671 6 View

Stable transfection be combined with two or more antibiotics to screen cells?

Can positive cell screening after stable transfection be combined with two or more antibiotics to screen cells? Will all the cells die?

10 June 2024 9,739 0 View

I want to ask if I suppose to add 200mg of antibiotic in 100ml of water so what is the concentration of this stock solution in microgram?

Hello, I recently started working with antibiotics and I do not have that much experience of making dilutions accurately. I want to ask if I suppose to add 200mg of antibiotic in 100ml of water...

08 June 2024 2,285 6 View

Should there be a difference in biotin management of Alopecia if the patient is a chronic energy drink user?

We have bumped into this energy drink, Celsius, with 1000% of RDA. I am starting to collect some information about this, but there is paucity of information.

08 June 2024 4,733 0 View

Is it okay to dissolve Ampicillin Anhydrous in 1 M NaOH?

So I made a 100 mg/ml solution of Ampicillin and in order to solubilize the powder I used 1 M NaOH. I noticed the solution turns yellow shortly after dissolving and I was wondering if some...

05 June 2024 7,170 0 View