Chris Taylor

5 Questions 2 Answers 0 Followers

Questions related from Chris Taylor

My DAB staining shows weak, sparse staining. What's wrong with my protocol?

I've been trying to stain some mouse brain sections for GFAP, but got some disappointing results. I can see that the antibody works since there are some visible astrocytes, but not much else. See...

07 January 2025 9,342 3 View

"Lines" in Raman Imaging acquisitions?

I'm using a InVia Renishaw Raman microscope system (785nm wavelength, 600l/mm grating density) to acquire Raman images on human brain samples, attached to MgF2 slides, at 50um resolution. The...

28 August 2024 5,515 1 View

Why do some of my Raman spectra show no signal at lower Raman shifts?

I'm using a InVia Renishaw Raman microscope system (785nm wavelength, 600l/mm grating density) to acquire Raman images on human brain samples, at 50um resolution. The samples are placed on MgF2...

31 July 2024 9,108 3 View

IHC secondary: does anti-chicken IgY ONLY bind to IgY primary antibodies?

I'm performing some DAB-enhanced anti-GFAP staining. My primary is polyclonal chicken anti-GFAP (AB5541): The antibody isotype is described as N/A in the SDS. We have a goat anti-chicken IgY...

15 April 2024 9,748 1 View

Removal of agar from vibratome sections?

I am sectioning human / mouse tissue on a vibratome. At both 50um and 20um, the sections float off in surrounding PBS still with the surrounding agar layer attached, and I can't seem to easily...

21 December 2023 6,370 2 View