Chris Taylor

3 Questions 2 Answers 0 Followers

Questions related from Chris Taylor

Why do some of my Raman spectra show no signal at lower Raman shifts?

I'm using a InVia Renishaw Raman microscope system (785nm wavelength, 600l/mm grating density) to acquire Raman images on human brain samples, at 50um resolution. The samples are placed on MgF2...

31 July 2024 8,822 3 View

IHC secondary: does anti-chicken IgY ONLY bind to IgY primary antibodies?

I'm performing some DAB-enhanced anti-GFAP staining. My primary is polyclonal chicken anti-GFAP (AB5541): The antibody isotype is described as N/A in the SDS. We have a goat anti-chicken IgY...

15 April 2024 9,739 1 View

Removal of agar from vibratome sections?

I am sectioning human / mouse tissue on a vibratome. At both 50um and 20um, the sections float off in surrounding PBS still with the surrounding agar layer attached, and I can't seem to easily...

21 December 2023 6,364 2 View