I have been dealing with this for 4 weeks now, i had perfect loading of protein samples and ladder but when doing the semi dry transfer, i stain the membrane with ponceau solution and see no protein samples and when i stain the gel the samples are still in there, i changes every single material used in western blot such as buffers, cassettes, electrode assembly, duration of transfer i made it longer for 1hour and 30 minutes also, tried the wet transfer and got similar results meaning no protein samples on both.