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03 March 2021 5,360 2 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Can part of recombinant protein not be expressed?

Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...

14 February 2021 2,039 4 View

Proteins not stacking/ entering in a native PAGE. Can anyone help?

Hi. I am trying to study a biotin modification of my target protein in a crude cell extract and am interested to determine how much of my protein in getting biotinylated. After biotinylating my...

15 January 2021 2,214 4 View

Co-stain Lectin PNA FITC conjugate with an uncojugated primary antibody?

Hello, I am planning to stain retinal tissue sections for IHC and want to co-stain cones and rods. For this we plan on using a Lectin PNA From Arachis hypogaea (peanut), FITC Conjugate for cones...

12 January 2021 583 1 View

Simultaneous imaging of multiple components (Carbohydrates, Fat, Protein) in food products using confocal microscopy??

Hi Everyone, I am trying to characterize the microstructure (localization of carbohydrates, fats, and proteins) of coconut milk powder using confocal microscopy. From the review of the available...

12 January 2021 5,464 2 View

What is the suitable date of FITC-DMSO solution using?

Hello I want to know if I make a FITC-DMSO solution, how long time I can use it? Can I use the solution after a week? thanks for your help

08 January 2021 1,506 3 View

Do polymers with different chain lengths have UV-Vis absorption peaks at different wavelengths?

I have a sample of mixtures containing polystyrene microspheres (PS-MS) with 3 different diameter sizes (bought from thermo), they all contain a green fluorescent dye inside the polymer matrix /...

06 January 2021 2,218 5 View

Is it ok to use mCHERRY(plasmd-tagged) and FITC(antibody-tagged) as a pair to test interactions between proteins by FRET-FLIM?

Can anyone please suggest to me whether I can able to check the fret efficiency between two proteins when one protein expressed after transfecting the cells, that might act as a donor(...

06 January 2021 6,958 3 View

What buffer to use for biotinylation as alternative to PBS?

I'm looking to biotinylate a protein that precipitates in PBS. The recommended reconstitution buffer for the protein is urea/Tris buffer, pH 7,5 (7 M urea, 5 mM EDTA, 15 mM mercaptoethanol, 20 mM...

04 January 2021 7,681 5 View