Why do my FITC streptavidin microsphere beads (Bangs laboratories) loose their fluorescence in an antibody binding assay?

Does anyone know where to find polypropylene microspheres or microparticles around 1-5micrometer?

These will be used for the study of microplastics in the lung

29 July 2024 9,609 2 View

How do I avoid HEK293 cell lifting during biotinylation?

I’m doing cell-surface biotinylation with HEK293 cells and after all the washing and biotin incubation steps (washing with PBS ph8, neutralizing unreacted biotin with 40mM Tris ph8) many of my...

23 July 2024 4,740 3 View

ELISA sandwich with two monoclonal antibodies ?

hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...

16 July 2024 9,044 10 View

Flow cytometry compensation with beads - is the matrix the problem?

I am trying to compensate my flow cytometry data. I have human tumor tissue, dissociated and stained with live/dead fixable::APC, EpCAM::FITC, CD45::PE. I also stained and measured single stain...

07 July 2024 5,614 5 View

Can FITC fluorescence stay in conjugated proteins after running a Western-Blot?

I have FITC labeled proteins, and I would like to detect its fluorescence after running proteins western blot. Does fluorescence quench in WB condition? so I can detect FITC labeling (without...

27 May 2024 4,087 3 View

What is best stabilizing buffer for conjogation of quantum dots to antibodies?

We are researching the conjugation of various antibodies to quantum dot microspheres and europium microspheres. The sustainability results have been unsatisfactory. Which stabilizing buffers do...

25 May 2024 7,663 3 View

What method is good for doing in vitro degradation study of PCL microsphere by change in weight calculation or change in diameter (from microscope)?

I have prepared some polymeric microspheres from chitosan and PCL. However while performing in vitro degradation study, I found difficulties in both above mentioned methods. Here I am mentioning...

20 May 2024 6,114 4 View

Is anybody experiencing problems with the Quantum™ Simply Cellular reagents from Bangs laboratories?

We cannot discriminate the brightest populations when coupled with FITC-labelled antibodies

14 May 2024 9,055 0 View

What are the prices for the following antibodies?

BD Pharmingen™ FITC Mouse Anti-Rat αβ T-Cell Receptor (561672) BD Pharmingen™ APC Mouse Anti-Rat CD4 (550057) BD Horizon™ V450 Rat anti-Mouse CD8a (560469) BD Pharmingen™ PE Mouse Anti-Rat CD45R...

10 May 2024 3,072 0 View

Can I mix TRITC-labeled dextran and FITC-labeled lectin for one injection instead of having to do two injections of each?

For my protocol, at end-point I have to do tail-vein injections of TRITC-labeled dextran (100 ml of 10 mg/ml) and FITC-labeled lectin (50 mg). However, the protocol I'm following states that they...

01 May 2024 8,090 1 View