Why do my FITC streptavidin microsphere beads (Bangs laboratories) loose their fluorescence in an antibody binding assay?

In my experiment, I've attached biotinylated spike protein to FITC streptavidin microsphere beads. When I run the beads solo in the Flow 20%) for FITC. Has anyone else had this issue with these...

08 December 2022 4,557 2 View