Dear all,

I am currently trying to isolate DNA from samples stored in RNAlater (-20°C) with a standard phenol-chloroform extraction protocole. Unlike other samples stored in EtOH, when I precipitate the DNA at the end of the extraction, I obtain a viscous pellet at the bottom of my tubes. This is obviously coming from the RNAlater, and although I washed my samples prior to the extraction with ddH2O, the final DNA amount and quality suffer greatly from it...

Has anybody experienced that before? And does anybody know how to get rid of it?

Thank you very much!

Alex