Can anyone recommend utilizing the plasmid extraction kit for extraction of the circular DNA genome of viruses associated with insect vectors?

Dear Colleagues, I am planning to detect and discover DNA viruses particularly those with a circular genome and transmitted by insect vectors. However, I am confused about selecting the best...

10 January 2021 9,534 4 View

Is there a certain way of making HEPES buffer w/ KCl?

I am trying to switch from NaCl to KCl in my buffer. As soon as I add 4M KCl solution to DI water and HEPES, it precipitates. Would heating the buffer alleviate the problem? Or should i add KCl to...

07 January 2021 1,104 1 View

What are some of the differences between a research article and a review article?

What are some of the differences between a research article and a review article? Thanks in advance for your replies!

29 December 2020 2,066 26 View

For experts in Gaussian 09 for windows?

Dear Friends: 64 bit Gaussian 09 software for windows is more expensive than 32-bit Gaussian 09. In our work, we deal with coordination compounds with about 50 atoms. So, can we use 32-bit...

27 December 2020 2,877 2 View

Amorphous XRD Data?

Dear colleagues, I analyzed my material using XRD analysis and the material has shown amorphous behavior , how can I specify the composition of the material is there any extra analysis I can...

24 December 2020 1,280 10 View

Suggestions for Journals related to Psychology and Computer Science?

I am looking for a journal, related to Psychology and Computer Science. IF < 2.0. If anyone has published some related work, or know any journal. that would be nice.

07 December 2020 1,277 3 View

FEM modeling of Joints/ Connection?

The attached Joint.png showed and example case of a joint (2 continuum members are connected). There are 3 characteristics of this, 1. There is a Gap: Cause small amount of rigid body rotation and...

07 December 2020 6,603 3 View

How to compute intermolecular interactions in mixture of solvent?

Hello Is it possible to compute intermolecular interactions in a mixture of solvent using DFT tools in Gaussian-09 or GAMESS US, DMOl3 or any other computational chemistry code? For example we...

05 December 2020 7,336 3 View

How can we set up Hydrothermal Liquefaction using solar/microgrid for extracting biofules?

Fo the conversion of biomass to biofuels, Hydrothermal Liquefaction is chosen.But the whole operation has to be from renewable energy source including the whole process most probably by solar and...

23 November 2020 9,432 2 View

Do you think that the emergence of CORONAVIRUS affects Investor trading behaviors? How? give examples?

any Thoughts on this question will help me Thanks

06 November 2020 7,654 4 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View