Hello
I've prepared 4 liters (at once) of c-SBF (simulated body fluid) according to the method described by Kokubo,,, but when I finished adding all the materials the pH was 1 instead of 7.5!
I've used to prepare 2 liters each time and multiplying the amount of each component by 2 and it was working good
Now I multiply it by 4 to prepare 4 liters...so why did that happened?
and how can I solve the problem???
The method of preparation is described in the paper below:
http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.10482/abstract
The original SBF solution, which can be named c-SBF, is a TRIS-HCl pH-buffered solution (pH = 7.40 at 37 ºC) that can be prepared as indicated at Table 5.1 (p. 206) of the following reference:
Thesis Cristalização de Biomateriais Vitrocerâmicos e Mineralização...
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