How to avoid ATP degradation while performing ATP assay?

I am using the ATP Assay Kit (Colorimetric/Fluorometric) (ab83355) for measuring intracellular ATP from treated cell lines. Although my standards are wokring fine when using the fluorometric...

15 February 2021 3,746 4 View

What is the time required for an enzyme or an protein molecule to consume an ATP molecule during a reaction?

In what time do a protein body consumes an ATP molecule for an active process to happen?

02 October 2020 8,135 4 View

What are the effects of phosphorus deficiency on dehydrogenase activity in soil bacteria and fungi?

I suspect that under phosphorus deficiency the electron transport chain in the plasmamembrane and mitochondria of soil bacteria and AM fungi, respectively, is disrupted as a result of lower ATP...

30 August 2020 4,659 3 View

Please recommend biological investigation of newly identified anticancer compounds that specifically inhibit Src kinase?

We aim to publish ta high impact journals (If 6+). Compounds showed incredible activities on cancer cell line (submicromolar on several types). I need your recommendation on how to determine...

24 May 2020 1,230 3 View

Monitoring ATP binding to proteins: TNP-ATP or MANT-ATP?

Hi all, I'm interested on monitoring ATP binding to a protein of interest using fluorescent ATP analogs, such as TNP or MANT-ATP. Do you have any experience with them? Which one is the best...

03 May 2020 3,993 5 View

ATP content measurement?

Hello, I am looking for a clear protocol for ATP content assay. I am not looking for a kit because it is expensive. I am looking for a fluorogenic or a colorimetric substrate that helps me to...

24 March 2020 2,286 3 View

ATP evoked calcium response in skin fibroblasts: no response?

23 March 2020 4,854 2 View

Does POM1 cause precipitation with ATP?

Hi, I've been doing multiple experiments to measure POM1 inhibition of ATP breakdown by CD39. I measure the amount of breakdown using either the malachite green assay or the ATP luciferase...

17 February 2020 5,340 1 View

Is ATP the best energy providing molecule? can there be something better?

ATP energy-storing molecule as is no doubt an efficient! as not only it is used as energy storage and provider in all forms of life and has probably been around for billions of years. Even in the...

08 February 2020 9,015 6 View

Is it possible to activate macrophages NLRP3 inflammasome without priming?

06 February 2020 6,888 4 View