I added 10mM or 20mM itaconic acid solution (PH7.0) into BMDM cells for 12h and then added LPS and ATP to induce the activation of NLRP3 inflammasome, but found that itaconate could not inhibit the activation of NLRP3 inflammasome
Hello everyone We are building an atomic structure model against a cryo-EM map of AAA+ ATPase. Considering that AAA+ polymerizes when ATP is added, we collected data for AAA+ mixed with ATP. Our...
04 April 2024 4,640 2 View
I am trouble shooting a malachite green ATPase assay using a kit from Sigma (MAK307) for a cytosolic protein that converts ATP --> ADP + Pi and am wondering what cheap + commercially available...
30 March 2024 9,659 1 View
Hello! I'm plannning to investigate the relationship between mitochondrial mass/function and epithelial homeostasis/differentiation. Are there any activators(drug) of mitochondria interms of...
27 March 2024 8,273 0 View
I need to order an ATP Assay Kit for my work, and I found this kit on the BT Lab website. Has anyone tried the kits from this site? Because this is the first time I will order from them.
27 February 2024 6,940 1 View
I am planning to activate inflammasome in macrophages and epithelial tumor cells. I was wondering if I could harvest macrophages right after nigericin treatment (for example 4 hours treatment then...
06 January 2024 490 0 View
Hello, I performed the ADP-Glo kinase assay. In that case, samples(active protein+substrate+ATP+Kinase buffer+w/wo test compound mixture) were incubated for 45 minutes in 30℃. In the next...
27 December 2023 8,915 2 View
I am working with HepG2 cells to observe effects of Simvastatin treatment on the cells. We are aiming to identify a dose response associated with levels of ATP, metabolic activity, and CoQ10...
29 November 2023 6,062 0 View
I am currently trying to develop an ATP concentration measurement protocol for my human cultured cells. However, I have found little to no detailed protocol for ATP extraction using TCA...
27 November 2023 7,980 1 View
Hi dear All. I'd like to know simplest protocol to detect ATP binding assay (ATP binding with protein). I read a couple of research about this but I want to know common methods. Thanks for your...
19 November 2023 7,412 0 View
process of human obtaining energy in cellular respiration
28 October 2023 7,303 1 View