Hello everyone,
My lab has been using CHO cells for electrophysiology for years and we have never had problems. Our cells used to look really good, go through transfection easily and stay stable during long patch clamp experiments.
Recently we started having problems, our cells look bad and many are apoptotic, those who aren't - do not express our DNA... Here is what we've already tried:
What else can we check? The problems started a few months ago and we thought it might be because of the proline, but using F-12 didn't change anything.
Thank you very much!
Are you putting the cells in your own 'labmade' solutions at any point. It sounds similar to a problem we had in the past that we eventually put down to low quality nanopure water?
E
Hi Mi, hope you have solved your problem. If not, do not reconsider everything at the same time but focus on what you may have changed. For instance, have you used a new serum from a different company? If not, are you still using the same batch or is it a new one?
- Badges
- Science topic
- Similar topics
- Engineering
- Petroleum Engineering
- Oil