Which western blot precentage and runnig time should I choose?

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Andrew Wilhelmsen

I would try making up a 20% gel, and running it for 60-90 minutes at a constant current https://www.novusbio.com/support/support-by-application/western-blot-sds-page

Mi La

Thank you.

Can you please explain the huge overlap between the protein sizes? How can they suggest different gel percentage for proteins 4-40kD or 12-45?