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Questions related from Mi La
Hello, I need to change two adjacent nucleotides. * I've added 5% DMSO * I've used an annealing temp that is 5 degrees higher than the tm of the primers. * Im using a hot start kit But after...
14 May 2020 1,071 3 View
Hello, I want to separate two purified proteins that have almost the same weight. One is 16.8 kDa, the other is approx 10kDa. I use Tris\glycine buffers. Which gel percentage and running time...
12 December 2018 5,599 2 View
Hello everyone, My lab has been using CHO cells for electrophysiology for years and we have never had problems. Our cells used to look really good, go through transfection easily and stay stable...
20 November 2018 6,733 3 View
Hello, When calculation the tm for gibson primers with an over hang of about 20bp, should I take into consideration the entire primer or just the part that anneals? or even some combination if the...
02 February 2018 464 4 View
Hello everyone. My question regards the mounting stage in the immunocytochemistry protocol. I use 10ul drops of Flouromount Aqueous Mounting (Sigma, kept at RT) to mount 13mm coverslips with fixed...
19 December 2017 7,290 5 View
Hello everyone, I need to prepare a 0.1% Triton x100 solution in PBS, but the Triton is too viscous so I dont know how much I'm using. Do you have any technique to liquefy it? Thank you!
15 June 2017 4,425 6 View
