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Questions related from Mi La
Hello, I need to change two adjacent nucleotides. * I've added 5% DMSO * I've used an annealing temp that is 5 degrees higher than the tm of the primers. * Im using a hot start kit But after...
14 May 2020 1,002 3 View
Hello, I want to separate two purified proteins that have almost the same weight. One is 16.8 kDa, the other is approx 10kDa. I use Tris\glycine buffers. Which gel percentage and running time...
12 December 2018 5,552 2 View
Hello, When calculation the tm for gibson primers with an over hang of about 20bp, should I take into consideration the entire primer or just the part that anneals? or even some combination if the...
02 February 2018 406 4 View
Hello everyone. My question regards the mounting stage in the immunocytochemistry protocol. I use 10ul drops of Flouromount Aqueous Mounting (Sigma, kept at RT) to mount 13mm coverslips with fixed...
19 December 2017 7,262 5 View
Hello everyone, I need to prepare a 0.1% Triton x100 solution in PBS, but the Triton is too viscous so I dont know how much I'm using. Do you have any technique to liquefy it? Thank you!
15 June 2017 4,396 6 View
