27 July 2018 1 4K Report

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I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Analysis of Tensile Test Results Without Using an Extensometer?

I conducted a tensile test to analyze the strength of a welded joint and to implement an FEM model. Due to the high hardness of the base material, slippage between the grip and the base material...

10 June 2024 5,736 6 View

Is there a application on multi-photon absorption materials?

Even though multi-photon absorption is fascinating phenomenon, recently I am agonizing about its applicability. Second harmonic generation is used in lasing materials or so, however, I have never...

28 April 2024 2,524 1 View

Protocol for neuraminidase treatment of adherent A549 cells?

Dear all, I am looking for a protocol for neuraminidase (NA) treatment of adherent A549 cells (as a control for SNA and MAL-II staining). I am particularly interested in: - Did you fix the...

22 April 2024 9,938 1 View

How do I prevent dark blue spots/smear and the running front of my BN-PAGE?

Hi everyone, we are trying to establish BN-gels in our lab and we are currently facing some problems. We would like to investigate protein-protein interaction of membrane bound proteins from cell...

18 April 2024 3,219 0 View

What is the principle of combining malachite green (MG) and crystal violet (CV) to gold nanoparticle (AuNP) for Raman (SERS) measurement?

It is said that substances such as malachite green (MG) and crystal violet (CV), which are used as Ramandye, are combined with Gold nanoparticle (AuNP) to measure Raman (SERS). I'm curious about...

05 February 2024 8,060 3 View

Difference between polaron and self trapped exciton?

what I've heard is the self-trapped exciton is another name of small polaron. But can I ask what the difference and definition of self-trapped exciton and polaron? Sincerely,

21 December 2023 4,135 0 View

How can I achieve uniform PR coatings on rectangular substrates?

I would like to achieve uniform PR(photoresistor) coatings as thin as 300 nm on a thick glass substrate. SU-8 PR is thinking of using with dilution. The size of the substrate is 160*120mm...

18 December 2023 4,457 2 View

How to Cell culture on PDMS without O2 plasma treatment?

I am a master's student in Korea. I have some questions while conducting a cell culture experiment on PDMS. 1. What should I do to allow HaCaT cells to grow on PDMS without O2 plasma treatment...

12 December 2023 5,557 0 View

How to Cell culture on PDMS without O2 plasma treatment?

I am a master's student in Korea. I have a question while conducting a cell culture experiment on PDMS. What should I do to allow HaCaT cells to grow on PDMS without O2 plasma treatment during...

12 December 2023 6,649 0 View

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Is this website real?

Is this website real? https://isar.org.in/event/registration.php?id=2434532

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Is it legal to upload a graphical abstract previously published on your paper's first page to a website such as figshare.com under CC-BY-4.0 license?

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