Why cannot we use genomic DNA isolated from mammalian cells for DNA -metal binding studies ?

05 May 2019 1 3K Report

I wanted to do UV -vis spectroscopy for DNA - metal binding study but I have found in most of the publications people have used CT-DNA. Why not isolate DNA from any cells and perform the assay ?

Michael J. Benedik

By CT-DNA I presume you mean calf thymus DNA? Cows are of course mammals so this is mammalian DNA. I think calf thymus has been a traditional source of DNA because one could get that tissue at slaughterhouses where cattle were butchered for meat, so it was a good source of tissue to make DNA and it was easy to extract DNA from the thymus.

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