Using phusion polymerase I get amplification but when I try using Taq I get no results.
What is size of your DNA fragment?? What is AT/GC %??
Some times size and GC % affect such reactions.
Have you check with repeat template preparation?
Phusion has way higher processivity and lower elongation time than Taq, so it is overall a more efficient enzyme.
It happens. size of the DNA fragment to be amplified matters a lot. Phusion is best choice for amplification of large DNA fragments
If you are cloning restriction sites onto the ends of your cloned gene then you can't use Taq as it has 5'-3' exonuclease activity, which will chew away the 5' ends of primer overhangs
High-Fidelity DNA Polymerase offers both high fidelity and robust performance. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. It has an error rate > 50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase. It is also most accurate thermostable polymerases which possesses 5´→ 3´ polymerase activity, 3´→ 5´ exonuclease activity and will generate blunt-ended products.
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