8 Questions 15 Answers 0 Followers
Questions related from Rothangmawi Victoria Hmar
If a compound shows two peaks in HPLC will it also show two absorption spectrum?
06 June 2019 5,757 6 View
I would like to know the list of nitrogen containing products that microbes (non-pathogen) usually produce
02 February 2016 4,877 0 View
I have carried out enzyme activity studies for strains A, B , C . Though the activity is different for all, the specific activity is almost same. So which data should I represent or will have more...
11 November 2014 4,363 7 View
If I want to prove that the half-life of my mRNA is low, what experiments should I do? I have the q-PCR data, can I determine the half-life from the data?
11 November 2014 3,203 4 View
I cloned a 4.2 kb gene in a vector. Restriction digestion confirmed the presence of the insert. When I carried out PCR with the primers of the gene I get a band but its only 3 kb. How is it...
05 May 2014 7,576 3 View
Using phusion polymerase I get amplification but when I try using Taq I get no results.
05 May 2014 2,773 5 View
I have a 2 kb gene coding for a transmembrane protein. When I clone this gene into plasmids in E.coli, I can never get the desired plasmid with insert. I have tried using several plasmids but it...
05 May 2014 3,171 16 View
All genes in an operon are under the control of the same promoter, so will there be difference in the expression of the genes? Are there any reports on this?
05 May 2014 1,104 9 View