Rothangmawi Victoria Hmar

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Questions related from Rothangmawi Victoria Hmar

What is the need for spectrum analysis? or what information is obtained?

If a compound shows two peaks in HPLC will it also show two absorption spectrum?

06 June 2019 5,756 6 View

What are some important nitrogen containing products produced by microbes?

I would like to know the list of nitrogen containing products that microbes (non-pathogen) usually produce

02 February 2016 4,876 0 View

Which data is more important- the activity of enzyme or the specific activity of enzyme?

I have carried out enzyme activity studies for strains A, B , C . Though the activity is different for all, the specific activity is almost same. So which data should I represent or will have more...

11 November 2014 4,362 7 View

How would I determine the stability of mRNA?

If I want to prove that the half-life of my mRNA is low, what experiments should I do? I have the q-PCR data, can I determine the half-life from the data?

11 November 2014 3,202 4 View

Why do I get a PCR band of the wrong size after cloning, though restriction digestion confirms the presence of the gene?

I cloned a 4.2 kb gene in a vector. Restriction digestion confirmed the presence of the insert. When I carried out PCR with the primers of the gene I get a band but its only 3 kb. How is it...

05 May 2014 7,575 3 View

Why can some DNA fragments/genes only be amplified by high-fidelity polymerases and not by Taq?

Using phusion polymerase I get amplification but when I try using Taq I get no results.

05 May 2014 2,772 5 View

Is there a possibility that certain DNA fragment / genes cannot be cloned in E. coli?

I have a 2 kb gene coding for a transmembrane protein. When I clone this gene into plasmids in E.coli, I can never get the desired plasmid with insert. I have tried using several plasmids but it...

05 May 2014 3,170 16 View

Will the genes of an operon be expressed differentially?

All genes in an operon are under the control of the same promoter, so will there be difference in the expression of the genes? Are there any reports on this?

05 May 2014 1,103 9 View