Hi, everyone!

This is my first question posted to Research Gate, so I hope I’m doing this right. Please correct me if I need to change anything in future posts.

I’ve attempted performing a viral plaque assay three times now without any plaques forming in any of the trials. I have little to no background in microbiology, so it’s possible that I’m making a simple and obvious mistake, but I’m open to any and all feedback. I am using the mammalian cell NCTC clone 1469 in a 6-well plate and infecting it with MHV. I’ve outlined the general procedure used below.

Is it possible that an Endpoint Dilution Assay would be more effective in determining viral concentration for this specific cell and virus combination?

Thank you!