Hi, i am working with a fluorescence spectrometer. The substances i'm examine are solved in ethanol. In addition to my measurements of a dilution series of a substance, I measure the pure ethanol in order to be able to subtract it later on. Although ethanol itself contains no fluorophores, I can still measure a spectrum of pure ethanol. A friend of mine who is in the pharmaceutical industry calls the obtained ethanol spectrum only a "blank value of the solvent". For me, however, the spectrum of ethanol seems very clear. Unfortunately, I could only find very limited literature on the fluorescence of ethanol, can anyone help me ? So why can I record an ethanol spectrum although ethanol is not a typical fluorescent substance in terms of structure? Which emission do I measure ? How is the spectrum of ethanol evaluated in general, simply as noise in the measurement ?
Thanks in advance.
Kind regards Meret