Does gold coated affect the EDX analysis?

I have some stubs of plant tissues that are gold-coated. I was wondering if there is a possibility to do EDS analysis. Does the energy of the K or L edge of gold affect the analysis of the lighter...

01 February 2019 8,293 7 View

What is the relationship between arabinoxylan and lignin?

I am studying the plant cell wall and I have a question concerning its chemical composition. There is a relationship between lignin and arabinoxylan. Can arabinoxylan help the lignin...

08 September 2016 6,575 9 View

Fungal elicitors can interfere in plant transcription factors?

I will start a work on plant-pathogen interaction and I want to know if biotrophic fungi can secret elicitors in order to modify the transcription factors in the host. I am reading some papers...

05 June 2016 1,564 5 View

What is the best mounting medium to study plant cell on real-time?

I am trying to study living plant cell on fluorescence microscopy. Should I use PBSor sucrose? Do you have some suggestions? Thanks

04 May 2016 8,088 4 View

Are chitinase genes expressed only in infected tissues?

I am studying the Chitinase expression profile in fungal infected tissues comparing with non-infected. Does anybody have some information about the chitinase expression profile against fungi? Does...

02 March 2015 5,432 3 View

What is the function of triethanolamine during the in situ hybridization? How does this reagent interacts with the RNA?

01 February 2015 5,645 8 View

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02 March 2021 3,060 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View