I am studying the Chitinase expression profile in fungal infected tissues comparing with non-infected.
Does anybody have some information about the chitinase expression profile against fungi? Does it is express only in infected tissues? What about healthy tissues?
Thank you
Chitinase gene expression is induced in response to fungal infection.Chitinase is often produced in higher plants as a general defence response after wounding or pathogenic attack.Chitinases accumulate in seeds of several species as part of their developmental programme, while others can be induced in response to microbial attack. Seed chitinases may protect against chitin-containing pathogenic fungi, because substrates for chitinase are found in some fungal cell walls, but not in plants
Chitinase can be found in trace amounts as a preformed compound even in healthy plant tissues. However, magnitude of expression is generally higher following pathogen attack or wounding.
Dear Ravi and Ganga,
I asked about chitinase expression because I am doing a in situ hybridiaztion experiment to localize chitinase transcripts in tissues infected with a fungal pathogen, but I do not found the positive signal in uninfected tissues. Does the absence of the signal in uninfected tissues is probably related to the abscense of Chitinase expression?
What do you think?
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