Why pyoverdine expression in P. aeruginosa is more inhibited in semi-solid media than the liquid media?

Hi colleges. I was working in inhibition of pyoverdine expression in P. aeruginosa and performed swarming inhibition tests in semi-solid media too. I saw the results of swarming inhibition on the...

11 December 2018 3,508 0 View

No title

09 October 2017 6,117 5 View

What is the stability of plant extracts in DMSO?

01 February 2015 544 4 View

Can we say that the kinetic antibodies in viral infections are the same in all viruses ?

Could we say that in all infection caused by virus or in all population, kinetic antibody response is equal?

02 March 2014 4,883 3 View

What is the difference between Gnaphalium polycephalum and Helichrysum italicum?

Apparently are similars

31 December 2013 8,926 2 View

What is the standardized protocol to obtain information about plant samples from ethnobotanical surveys?

I need a protocol to obtain information about plant samples from an ethnobotanical survey?

09 October 2013 7,407 6 View

Is tumbling true bacterial motility?

Tumbling

09 October 2012 8,990 10 View

What's the difference between Kovat's Retention Index and Linear Retention Index

01 January 1970 1,725 0 View

Collegues, someone have a protocol (without automatization) in order to do antibacterial effect assay of nat products in vivo with C. elegans model?

01 January 1970 5,169 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What are the most relevant MSc proposal topics for Petroleum Production Engineering at the moment?

I am a Petroleum Production Engg MSc student at Robert Gordon University in Aberdeen and I am in the process of selecting a topic for my MSc Engineering Investigation proposal. I would very much...

01 March 2021 7,396 4 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View