Collegues, someone have a protocol (without automatization) in order to do antibacterial effect assay of nat products in vivo with C. elegans model?

More Román Y Ramírez-Rueda's questions See All

Why pyoverdine expression in P. aeruginosa is more inhibited in semi-solid media than the liquid media?

Hi colleges. I was working in inhibition of pyoverdine expression in P. aeruginosa and performed swarming inhibition tests in semi-solid media too. I saw the results of swarming inhibition on the...

11 December 2018 3,609 0 View

What should be the concentration of isolated compound in terms of µM to consider this as a hit in an antimicrobial screening microdilution assay?

What should be the concentration of isolated compound in terms of µM to consider this as a hit in an antimicrobial screening microdilution assay? And what should be the concentration of this...

09 October 2017 6,217 5 View

Why C. violaceum wild type (ATCC 31532) loose spontaneously pigment production?

How can I recover pigment production in this strain? Thanks for your answers.

01 February 2017 9,742 2 View

What is the stability of plant extracts in DMSO?

We have working with plant extracts in order to bacterial inhibition

01 February 2015 698 4 View

Can we say that the kinetic antibodies in viral infections are the same in all viruses ?

Could we say that in all infection caused by virus or in all population, kinetic antibody response is equal?

02 March 2014 5,006 3 View

What is the difference between Gnaphalium polycephalum and Helichrysum italicum?

Apparently are similars

31 December 2013 9,074 2 View

What is the standardized protocol to obtain information about plant samples from ethnobotanical surveys?

I need a protocol to obtain information about plant samples from an ethnobotanical survey?

09 October 2013 7,559 6 View

Is tumbling true bacterial motility?

Tumbling

09 October 2012 9,143 10 View

What's the difference between Kovat's Retention Index and Linear Retention Index

Hi friends. I'm working with essential oils and I have doubt of how I must to report the retention index of compounds identified by GC-MS. Thanks for your replies!!

01 January 1970 1,839 0 View

The Bigger You Are, the Harder You Fall (some lessons from Dinosaurs)?

Evolutionary fitness is based on an organism’s ability to adapt rapidly to changing environmental circumstances. Large-bodied mammals have been equipped with large brains (and hence a high...

06 August 2024 4,849 2 View

Are air moisture harvesting technologies effective in combating desertification?

Air moisture harvesting Air water collection devices

06 August 2024 5,473 2 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

State of art in natural disasters?

Are increasing the costs of disasters in the affected countries.

01 August 2024 1,794 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Posthoc test lettering in JAMOVI?

Does anyone know of a module for the JAMOVI software that is capable of generating mean separations using the classic letters based on post hoc results (e.g., Tukey test)? If, as I believe, such...

31 July 2024 3,333 4 View

What are the roles of innovation in achieving the Sustainable Development Goals (SDG)?

31 July 2024 3,533 2 View

Culture plates for PBMC (non-adherent). For infection?

Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...

24 July 2024 2,158 2 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...

23 July 2024 8,492 3 View

What's technologies for enhancing crop productivity in dry-land areas & agro technologies for sustainable development of rain fed & water scarce area?

What are the technologies for enhancing the crop productivity in dry-land areas and agro technologies for sustainable development of rain fed and water scarce area?

21 July 2024 2,964 4 View