I am trying to assess BTB integrity. Most articles show experiments with Biotin Tracer. But incase of Blood brain barrier Evans blue or albumin is used often. Why in case of BTB biotin tracer is used more often?
Dear Rabia,
in my opinion, it is due to easy detection of the Biotin Tracer.
Best regards
Pavel
Thank you so much Dr. Travnik. I was just wondering if there is any relation to the location of the blood vessels in the interstitium of the testis.
Perhaps Biotin (MW about 250) is used because it is a lot smaller that Evans Blue (MW about 950) or albumin (big sticky protein)
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