Why aromatic imines are weakly fluorescent?

More Lucas Fonseca Bezerra's questions See All

What is the relationship between the pi-sextets and the absorption spectrum of a polyciclic aromatic hydrocarbon?

Could anyone suggest me some papers to get a better understanding of Clars model and UV-Vis spectroscopy ?

31 December 2018 5,749 2 View

Can anyone sugest me some articles to get familiar with Theoretical chemistry?

I want to get underground knowledge about this topic and more specifically DFT method.

04 May 2018 3,414 10 View

Can I attribute sensitizer luminescence quenching caused by oxygen to singlet oxygen generation?

Molecular oxygen can quench luminescence signal of fluorophores. I want to know if I can attribute this phenomenon only to singlet oxygen generation in a organic media. I plan to perform...

03 April 2018 8,064 3 View

Is there any singlet oxygen sensor that can be used under UV irradiation?

I want to measure singlet oxygen generated by organic molecules under blue or UV light irradiation, however the most comon sensor DPBF undergoes photochemical degradation in this condition and I...

03 April 2018 7,828 3 View

Can anyone help me in a syntheses of an aromatic imine (schiff base)?

I'm trying to react a aromatic aldehyde with the blue toluidine dye wich has a NH2 group atached on the aromatic ring. I performed it in methanol with molecular sieves adding 5 drops of acetic...

01 February 2018 6,902 5 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View