Hi,
We are using TaqMan advanced assay for miRNA. In one of the probes we have purchased a sample without RNA added to the reverse transcription reaction gives Ct just ~ 5 cycles higher that an average sample with cDNA. To exclude contamination all solutions were changed and the same result was obtained by three different lab members. Any ideas if this issue can be resolved?
Laurence Stuart Dawkins-Hall
- That tricky with pre optimised TaqMan probes
- I would approach the company about a replacement
- They will probably honour that if you provide evidence
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Natalia Volinsky
Thank you Laurence Stuart Dawkins-Hall ,
I already contacted the company, but was hopping to resolve this issue without a replacement.
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